D E F I N I T I O N S
The definitions of different parameters of the comet are dependent on the software one is using:
For KometTM software the definitions are as follows
Tail Length is the distance of DNA migration from the body of the nuclear core
and it is used to evaluate the extent of DNa
Moment: Tail moment is defined as the product of the tail length and
the fraction of total DNA in the tail. Tail moment incorporates a measure of
both the smallest detectable size of migrating DNA (reflected in the comet tail
length) and the number of relaxed / broken pieces (represented by the intensity
of DNA in the tail).
Head % DNA = (Head.OptInten /(Head.OptInten + Tail.OptInten)) X 100.
Tail % DNA = 100 - Head%DNA.
For Vis CometTM software the definitions are as follows:
Comet total intensity
algorithm starts from the beginning of the tail and examines each vertical scan
line until the end of the tail is reached. Each vertical scan line is processed
from top to bottom and only those pixels are considered that are identified as
each vertical scan line the product of breadth, position and total intensity is
integrated intensity is calculated as
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Tail Breaks Number:
Courtesy: Dr. N.P. Singh